Brand:aa
Product detail:
Name:Lysozyme
Synonyms:LYZ; muramidase; N-acetylmuramide glycanhydrolase
Molecular Formula:C36H61N7019
Molecular Weight:895.9134
CAS Number: 12650-88-3
EINECS: 235-747-3
The enzyme functions by attacking peptidoqlycans (found in the cell walls of bacteria, especially Gram-positive bacteria) and hydrolyzing the glycosidic bond that connects N-acetymuranic acid with the fourth carbon atom of N-acetylqucosamine. It does this by binding to the peptidoqlyucan molecule in the binding site within the prominent cleft between its two domains. This causes the substrate molecule to adopt a strained conformation similar to that of the transition state. According to Phillips-Mechanism the lysozyme binds to a hexasaccharide. The lysozyme then distorts the 4th sugar in hexasaccharide (the D ring) into a half-chair conformation. In this stressed state the glycosidic bond is easily broken.
The amino acid side chains glutamic acid 35 (Glu35) and aspartate 52 (Asp52) have been found to be critical to the activity of this enzyme. Glu35 acts as a proton donor to the glycosidic bond, cleaving the C-O bond in the substrate, whilst Asp52 acts as a nucleophile to generate a glycosyl enzyme intermediate. The glycosyl enzyme intermediate then reacts with a water molecule, to give the product of hydrolysis and leaving the enzyme unchanged.
Items | Standards |
Source | Hen egg white |
Appearance | Dialyzed white amorphous powder |
Moisture | 8.0% Max. |
Ash | 2.0% Max. |
pH Value | 3.0-4.5 |
Nitrogen | 16% Min. |
Solubility | 95% Min. |
Activity | 20000u/mg Min. |
Total Plate Count | 1000CFU/g Max. |
Yeast & Mold | 10CFU/g Max. |
Storage:Protect sunshine and rain, cool and dry. No Smoking. Do not transport them together with Poisonous and harmful goods
Package: 25kg.per drum
Shelf life:2 years
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